Biology of Disease Essay Example

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Biology of Disease

Inhibition of Growth of Breast Cancer Derived Cell Lines

(Q1) -Why do SMTKI (small molecule tyrosine inhibitors) not work on the HER3 receptor? Select one:

a. The HER3 receptor cannot bind ligands

b. The HER3 receptor does not undergo gene amplification in breast cancer

c. The HER3 receptor forms homodimers with HER2

d. The HER3 receptor has no kinase activity

e. The HER3 receptor is expressed at high levels in some breast cancers

(Q2) -The HER2 receptor is: Select one:

a. Overexpressed and frequently amplified in approximately 20% breast cancers

b. None of the above

c. Amplified but not overexpressed in approximately 20% breast cancers

d. Overexpressed and frequently amplified in approximately 80% breast cancers

e. Overexpressed but not amplified in approximately 20% breast cancers

(Q3) -Which of the breast cancer cell lines contained the highest number of HER2 receptors? Select one:

a. MDA-MB-361 and MDA-MB-453

b. SKBR3 and HEK 293/HER3

c. MDA-MB-3614

d. SKBR3 and MDA-MB-361

(Q4) -The mechanism of resistance to treatment with Herceptin involves: Select one:

a. All of the factors suggested here

b. Upregulation of HER3

c. The interaction of the ligand neuregulin 1 with HER3

d. Activation of alternative signalling pathways

e. Translocation of intracellular HER3 to the plasma membrane

(Q5) -If the rabbit anti-mouse Alexa Fluor 546 antibody is the secondary antibody which species was the SPG1 primary antibody raised in? Select one:

d. Rabbit

(Q6) -What concentration of NRG1b was required to stimulate the maximum amount of growth in MDA-MB-453 cells? Select one:

a. 0.01nM

(Q7) -In figure 3A (panel 3) cells transfected with HER2-YFP (yellow fluorescent protein) were labelled with Alexa Fluor 568 labelled Herceptin (Blackburn et al. 12). These cells were not permeabilsed. Does this labelling represent: Select one:

a. Endoplamic recticular staining

b. Plasma membrane staining

c. All of the above

d. Nuclear staining

e. Golgi staining

(Q8) -SKBR3 breast cancer cells were treated with 0.1lg/ml (0.66 nM) Herceptin. At what concentrations of the anti-HER3 antibody, SGP1 was significant inhibition of growth observed compared to treatment only with Herceptin? Select one:

a. 0.01ug and 0.1ug

b. 0.1ug and 1ug

c. 1ug and 10ug

d. 0.1ug and 10ug

e. 0ug and 0.1ug

Works Cited

Blackburn, Edith, et al. «A monoclonal antibody to the human HER3 receptor inhibits

Neuregulin 1-beta binding and co-operates with Herceptin in inhibiting the growth of

breast cancer derived cell lines.» Breast cancer research and treatment 134.1 (2012): 53-